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Hasegawa Co Ltd germline specific polycomb protein scml2

( A ) X-Y axis length measurements in late pachytene (RA13) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes. Green and blue dashed lines show X and Y chromosome axes, respectively. (c–e) Measured lengths of the X axis (c), Y axis (d), and the calculated Y/X axis length ratio (e) in late pachytene Sync-Spdya fl/fl cells and Sync-Spdya cKO cells with full Y-X NH synapsis . Each dot represents a single nucleus. ( B ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for γH2AX (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the γH2AX area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside γH2AX area in Sync-Spdya cKO spermatocytes, based on the fluorescence intensity measurements of Y loops inside and outside the γH2AX domain. ( C ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for <t>SCML2</t> (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the SCML2 area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside SCML2 area in Sync-Spdya cKO spermatocytes. ( D ) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes immunostained for FK2 (green), SYCP3 (red) and ACA (light blue) showing the absence of FK2 signals in the full Y-X NH synapsis region (dashed circle in (b)) of X-Y chromosomes in Sync-Spdya cKO spermatocytes. Scale bars, 2 µm. (c) Percentage of late pachytene Sync-Spdya cKO spermatocytes lacking FK2 signals at the full Y-X NH synapsis region. ( E ) Illustration of a proposed mechanism underlying SpdyA-mediated X-Y loop-axis organization at late pachynema. Data information: All values are presented as the mean ± SD. “ n ” represents the total number of pachytene spermatocytes scored per genotype: from two mice in ( A – C ), and from three mice in ( D ). Statistical analyses were performed using Mann–Whitney test, and both exact P values in ( A -d) and ( A- e) ˂ 0.000000000000001. .

Germline Specific Polycomb Protein Scml2, supplied by Hasegawa Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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germline specific polycomb protein scml2 - by Bioz Stars, 2026-09

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1) Product Images from "Speedy A governs non-homologous XY chromosome desynapsis as a unique prerequisite for XY loop-axis organization"

Article Title: Speedy A governs non-homologous XY chromosome desynapsis as a unique prerequisite for XY loop-axis organization

Journal: The EMBO Journal

doi: 10.1038/s44318-025-00528-8

Figure Legend Snippet: ( A ) X-Y axis length measurements in late pachytene (RA13) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes. Green and blue dashed lines show X and Y chromosome axes, respectively. (c–e) Measured lengths of the X axis (c), Y axis (d), and the calculated Y/X axis length ratio (e) in late pachytene Sync-Spdya fl/fl cells and Sync-Spdya cKO cells with full Y-X NH synapsis . Each dot represents a single nucleus. ( B ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for γH2AX (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the γH2AX area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside γH2AX area in Sync-Spdya cKO spermatocytes, based on the fluorescence intensity measurements of Y loops inside and outside the γH2AX domain. ( C ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for SCML2 (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the SCML2 area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside SCML2 area in Sync-Spdya cKO spermatocytes. ( D ) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes immunostained for FK2 (green), SYCP3 (red) and ACA (light blue) showing the absence of FK2 signals in the full Y-X NH synapsis region (dashed circle in (b)) of X-Y chromosomes in Sync-Spdya cKO spermatocytes. Scale bars, 2 µm. (c) Percentage of late pachytene Sync-Spdya cKO spermatocytes lacking FK2 signals at the full Y-X NH synapsis region. ( E ) Illustration of a proposed mechanism underlying SpdyA-mediated X-Y loop-axis organization at late pachynema. Data information: All values are presented as the mean ± SD. “ n ” represents the total number of pachytene spermatocytes scored per genotype: from two mice in ( A – C ), and from three mice in ( D ). Statistical analyses were performed using Mann–Whitney test, and both exact P values in ( A -d) and ( A- e) ˂ 0.000000000000001. .

Techniques Used: Staining, Fluorescence, MANN-WHITNEY

Cell Culture:

Article Title: Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm
Article Snippet: next wanted to examine whether bivalent marks are regulated at Class I regions during spermatogenesis. .. To this end, we focused on germline-specific Polycomb protein SCML2, which has a high affinity to hypomethylated DNA and is a critical regulator of germline transcriptomes and bivalent chromatin ( ; Maezawa, Hasegawa, Alavattam, et al., 2018; ; Maezawa, Yukawa, et al., 2018). .. SCML2 binding sites in spermatogonia predict the sites of H3K27me3 deposition in late spermatogenesis, an

Article Title: Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis
Article Snippet: Alavattam KG, Andreassen PR, Vidal M, Koseki H, Barski A & Namekawa SH 2017 Polycomb directs timely activation of germline genes in spermatogenesis. .. Genes and Development 31 1693–1703. (https://doi.org/10.1101/ gad.302000.117) Maezawa S, Hasegawa K, Yukawa M, Kubo N, Sakashita A, Alavattam KG, Sin HS, Kartashov AV, Sasaki H, Barski A et al. 2018a Polycomb protein SCML2 facilitates H3K27me3 to establish bivalent domains in the male germline. .. PNAS 115 4957–4962. (https://doi.org/10.1073/ pnas.1804512115) Maezawa S, Hasegawa K, Alavattam KG, Funakoshi M, Sato T, Barski A & Namekawa SH 2018b

RNA Sequencing:

Image Search Results

Journal: The EMBO Journal

Article Title: Speedy A governs non-homologous XY chromosome desynapsis as a unique prerequisite for XY loop-axis organization

doi: 10.1038/s44318-025-00528-8

Figure Lengend Snippet: ( A ) X-Y axis length measurements in late pachytene (RA13) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes. Green and blue dashed lines show X and Y chromosome axes, respectively. (c–e) Measured lengths of the X axis (c), Y axis (d), and the calculated Y/X axis length ratio (e) in late pachytene Sync-Spdya fl/fl cells and Sync-Spdya cKO cells with full Y-X NH synapsis . Each dot represents a single nucleus. ( B ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for γH2AX (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the γH2AX area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside γH2AX area in Sync-Spdya cKO spermatocytes, based on the fluorescence intensity measurements of Y loops inside and outside the γH2AX domain. ( C ) Immuno-FISH staining of late pachytene Sync-Spdya fl/fl (a–a″) and Sync-Spdya cKO (b–b″) spermatocytes for SCML2 (light blue), Chr Y (green) and SYCP3 (red). The white dashed line-enclosed area indicates Y chromatin signals located outside the SCML2 area. Scale bars, 2 µm. (c) Percentage of Y chromatin loop signals outside SCML2 area in Sync-Spdya cKO spermatocytes. ( D ) Sync-Spdya fl/fl (a) and Sync-Spdya cKO (b) spermatocytes immunostained for FK2 (green), SYCP3 (red) and ACA (light blue) showing the absence of FK2 signals in the full Y-X NH synapsis region (dashed circle in (b)) of X-Y chromosomes in Sync-Spdya cKO spermatocytes. Scale bars, 2 µm. (c) Percentage of late pachytene Sync-Spdya cKO spermatocytes lacking FK2 signals at the full Y-X NH synapsis region. ( E ) Illustration of a proposed mechanism underlying SpdyA-mediated X-Y loop-axis organization at late pachynema. Data information: All values are presented as the mean ± SD. “ n ” represents the total number of pachytene spermatocytes scored per genotype: from two mice in ( A – C ), and from three mice in ( D ). Statistical analyses were performed using Mann–Whitney test, and both exact P values in ( A -d) and ( A- e) ˂ 0.000000000000001. .

Article Snippet: The germline-specific Polycomb protein SCML2 (Sex comb on midleg-like 2) is recruited to X-Y chromatin at the early-to-mid-pachytene transition, suppressing the ubiquitination of H2A Lysine 119 (K119) on the X-Y chromosomes (Hasegawa et al, ; Luo et al, ).

Techniques: Staining, Fluorescence, MANN-WHITNEY

(A) Mononucleosome enrichment in spermatozoa at genomic sites of MethylCap-seq peaks of KIT + spermatogonia. Class I (green): genomic sites that are demethylated at the mitosis-to-meiosis transition, and Class II (red): all other sites. (B) H3.3 enrichment in the two classes of genomic sites in sperm. (C) Enrichment of H3.3 and nucleosomes (D) Enrichment of SCML2 in cultured germline stem (GS) cells, and H3.3 enrichment in sperm (left) and mononucleosome enrichment in sperm (right) (E) Enrichment of H3K27me3 and H3K4me3 during spermatogenesis and in spermatozoa. (F) RNA-seq analysis in embryos for three groups of genes. Top 1,000 nucleosome-enriched genes among class I and II peak-containing genes at promoters (7,577 and 7,261 genes) and bottom 3,000 nucleosome-enriched genes (Nucleosome-depleted genes).

Journal: bioRxiv

Article Title: Site-specific DNA demethylation during spermatogenesis presets the sites of nucleosome retention in mouse sperm

doi: 10.1101/2025.01.10.632457

Figure Lengend Snippet: (A) Mononucleosome enrichment in spermatozoa at genomic sites of MethylCap-seq peaks of KIT + spermatogonia. Class I (green): genomic sites that are demethylated at the mitosis-to-meiosis transition, and Class II (red): all other sites. (B) H3.3 enrichment in the two classes of genomic sites in sperm. (C) Enrichment of H3.3 and nucleosomes (D) Enrichment of SCML2 in cultured germline stem (GS) cells, and H3.3 enrichment in sperm (left) and mononucleosome enrichment in sperm (right) (E) Enrichment of H3K27me3 and H3K4me3 during spermatogenesis and in spermatozoa. (F) RNA-seq analysis in embryos for three groups of genes. Top 1,000 nucleosome-enriched genes among class I and II peak-containing genes at promoters (7,577 and 7,261 genes) and bottom 3,000 nucleosome-enriched genes (Nucleosome-depleted genes).

Article Snippet: To this end, we focused on germline-specific Polycomb protein SCML2, which has a high affinity to hypomethylated DNA and is a critical regulator of germline transcriptomes and bivalent chromatin ( ; Maezawa, Hasegawa, Alavattam, et al., 2018; ; Maezawa, Yukawa, et al., 2018).

Techniques: Cell Culture, RNA Sequencing

Genomic regions that are demethylated during the transition from mitotic spermatogonia to meiotic spermatocytes acquire H3K4me3 in meiotic spermatocytes, leading to SCML2-mediated deposition of H3K27me3, thereby establishing persisting bivalent marks at these hypomethylated nucleosome retention sites in spermatozoa.